Mejeeji DNA ati kolaginni RNA da lori ilana ilana iṣelọpọ-pipe ati kemistri phosphoramidite, a le lo synthesizer DNA lati ṣajọpọ RNA tabi awọn analogues RNA laisi iyipada siwaju, ati awọn reagents ninu iṣelọpọ DNA le ṣee lo taara ni RNA ati awọn acid nucleic atọwọda. ' kolaginni.Gẹgẹbi a ṣe han ni Nọmba 1, 2'-hydroxy ni RNA phosphoramidite ni aabo pẹlu ẹgbẹ aabo silyl, ie t-butyldimethylsilyl (TBDMS), idilọwọ awọn aati ẹgbẹ lori ẹgbẹ 2'-hydroxy ẹlẹgẹ.Ẹgbẹ TBDMS olopobobo ṣe idilọwọ iṣesi laarin phosphoramidite ati ẹgbẹ 5'-hydroxy lori atilẹyin to lagbara, ati pe akoko isọpọ to gun ni a nilo lati ni itẹlọrun ṣiṣe imudarapọ.
Iru bi DNA kolaginni, awọn synthesized RNA wa ni akọkọ cleavaged lati awọn ri to support nipa aminolysis, ki o si awọn TBDMS ẹgbẹ ti wa ni cleavaged nipasẹ tetrabutylammonium fluoride (TBAF) tabi trimethylamine trihydrofluoride.Robi RNA le di mimọ nipasẹ atunlo lati oti ati HPLC.
Nọmba 1. Ilana kemikali ti awọn ohun amorindun ipilẹ ni DNA ati RNA kolaginni.
a) dABz phosphoramidite ati b) rABz 2'-OTBDMS phosphoramidite.
Idagbasoke oogun siRNA nilo awọn afọwọṣe igbekalẹ ti RNA abinibi lati mu biocompatibility pọ si, 2'-hydroxy lori RNA le rọpo nipasẹ MeO, F ati ẹgbẹ MOE, ati acid nucleic titii (LNA) tun funni ni iṣẹ ṣiṣe to dara ni itọju ailera RNA. (Aworan 2).Awọn phosphoramidites wọnyi funni ni awọn iṣẹ ṣiṣe ti o jọra bi DNA iru phosphoramidites ninu iṣelọpọ, ati iṣẹ-si oke ati ilana isọdi ti awọn acids nucleic ti kii ṣe adayeba jẹ iru bi DNA abinibi.
Nọmba 2. Ilana kemikali ti awọn bulọọki ile ni awọn oogun siRNA.a) dABz 2-MeO phosphoramite;b) dABz 2-F phosphoramite;c) dABz 2-MOE phosphoramidite ati d) dABz Titiipa phosphoramidite.
Phosphoramiditesjẹ pataki fun iṣelọpọ ti awọn Jiini, pẹlu nipataki awọn idile DNA ati awọn idile RNA ati awọn itọsẹ wọn, gbogbo phosphoramidite loke ti a le funni ni package kan.
A n ṣe ilọsiwaju iṣẹ ti awọn ọja wa nigbagbogbo, ni pipe ilana iṣelọpọ wa ati gbigba awọn alaye ni ẹtọ.A ko fun ọ ni awọn ọja to gaju nikan, ṣugbọn tun fun ọ ni ikẹkọ ati iṣẹ.A ti ni ipese pẹlu oṣiṣẹ iṣakoso ọjọgbọn ati ẹgbẹ imọ-ẹrọ to dara julọ ati oṣiṣẹ itọju lati pese awọn alabara wa pẹlu iṣẹ pipe lẹhin-tita.
Akoko ifiweranṣẹ: Oṣu Kẹjọ-09-2022