Oligo ìwẹnumọ equipmnt fun Oligo alakoko kolaginni
Ohun elo ìwẹnumọ Oligo fun iṣelọpọ alakoko Oligo,
oligo kolaginni support ẹrọ,
Sipesifikesonu
| Awọn igbimọ ibaramu | 1, 3, 5, 8. |
| Sisẹ | fe ase, afamora ase |
| Nọmba awọn ibudo abẹrẹ | 5, 6, 7, 8, 9, 10. |
| Ibamu awo orisi | C18 awo, jin kanga awo, sintetiki awo (ibaramu pẹlu julọ sintetiki farahan), microtiter awo |
| Modulu | ẹyọkan tabi ipo meji |
| Foliteji | 220V |
| Atilẹyin ọja | 1 odun |
| Aṣa | Ti gba |
Oriṣiriṣi awọn ọna ti ìwẹnumọ
1. Gel Electrophoresis Chromatography ìwẹnumọ
Lo denaturing polyacrylamide gel electrophoresis chromatography fun ìwẹnumọ.Aṣoju denaturing ni gbogbogbo 4M formamide tabi urea 7M, ifọkansi ti acrylamide wa laarin 5-15%, ati ipin ti methacrylamide jẹ akọkọ laarin 2-10%.
Lẹhin electrophoresis, ipo ti ẹgbẹ nucleic acid nilo lati pinnu labẹ itanna ti ina ultraviolet, jeli ti o ni ibi-afẹde nucleic acid ti ge kuro, acid nucleic ti fọ ati jo, ati lẹhinna ojutu leaching ti wa ni idojukọ, desalted, iwon ati lyophilized.
2. DMT-Lori, HPLC ìwẹnumọ
Yan ipo DMT-Lori lakoko iṣelọpọ, ọja robi ti jẹ centrifuged o si dojukọ ni iwọn otutu yara lati yọkuro amonia pupọ lẹhin aminolysis.
Iyapa ti a ṣe pẹlu lilo iwe C18 pẹlu acetonitrile ati 10% triethylamine-acetic acid (TEAA) bi eluent.Lẹhin ti elution ti pari, o wa ni idojukọ, lẹhinna a ti yọ ẹgbẹ DMT kuro pẹlu trifluoroacetic acid.Lẹhin imukuro, diẹ ninu awọn iyọ ati awọn ohun elo kekere ni a yọ kuro nipasẹ tube ti a ge kuro, ati nikẹhin desalted.
Ọna yii le gba ọja pẹlu mimọ ti o ga julọ, ṣugbọn o jẹ dandan lati fiyesi si iṣẹlẹ ti depurination.
3. DMT-Pa, HPLC ìwẹnumọ
Yan DMT-Paa lakoko iṣelọpọ, ati pe ọja robi naa jẹ centrifuged ati pe o ni idojukọ ni iwọn otutu yara lati yọkuro amonia pupọ lẹhin ammonolysis.
Iyapa ti gbe jade nipa lilo iwe C18 pẹlu acetonitrile ati 10% triethylamine-acetic acid ninu omi bi eluent.Lẹhin ti iyapa naa ti pari ati iwọn, awọn aliquots ti wa ni lyophilized.
Ọna yii nilo atunṣe iṣọra ti awọn ipo iyapa, ati pe o tun le gba awọn ohun elo ibi-afẹde mimọ.
oligo synthesis accerry equipment for dna rna synthesis cycle, eyi ti o baamu fun deede alakoko, ati awọn miiran kukuru pq alakoko, ṣe awọn oligo ga ti nw.
olupilẹṣẹ china ti ẹrọ iṣelọpọ oligo pẹlu didara to dara ati iṣẹ to dara julọ.
