Ninu DNA aṣoju, RNA ati iṣelọpọ awọn acids nucleic ti kii ṣe ti ẹda, Idabobo ati Igbesẹ Isopọpọ ṣe awọn ipa pataki.
Igbesẹ Idaabobo ni lati yọ ẹgbẹ DMT kuro lori atilẹyin ti o lagbara tabi ẹgbẹ 5' hydroxyl lori nucleoside ti tẹlẹ pẹlu Organic acid, ati ki o ṣafihan ẹgbẹ hydroxyl fun igbesẹ isọpọ atẹle.3% trichloroacetic acid ni dichloromethane tabi toluene jẹ lilo pupọ julọ lati ṣe igbesẹ idabobo naa.Ifojusi ti trichloroacetic acid ati akoko idabobo (akoko deblocking) jẹ gaba lori mimọ ti awọn ọja ikẹhin.Idojukọ kekere ati aipe akoko deblocking fi ẹgbẹ DMT ti ko ni idahun, ti o dinku ikore ati mu awọn aimọ ti ko fẹ.Akoko didi gigun le yorisi depurine ti awọn ilana ti a ti ṣopọ, ti o dagba awọn airotẹlẹ airotẹlẹ.
Igbesẹ Isopọpọ jẹ ifarabalẹ si akoonu omi ti awọn olomi ati ọrinrin ninu afẹfẹ.Ifojusi ti omi ninu iṣelọpọ yẹ ki o kere ju 40 ppm, o dara julọ kere ju 25 ppm.Lati tọju ipo iṣelọpọ anhydrous, iṣelọpọ awọn acids nucleic yẹ ki o ṣe ni agbegbe ọriniinitutu kekere, nitorinaa a ṣeduro alabara wa lati loOhun elo Tituka Amiites, eyi ti o le tu lulú tabi ororo Phosphoramidite ni acetonitrile anhydrous lati yago fun olubasọrọ pẹlu afẹfẹ.
Niwọn igba ti tu ti awọn phosphoramidites o dara julọ ni ipo ti kii ṣe omi, ati awọn ẹgẹ molikula lati ṣe adsorb omi itọpa ninu awọn reagents ati amidite, o nilo lati mura silẹ.Awọn Ẹgẹ Molikula.A ṣeduro 2 g subsieve fun awọn igo reagent 50-250ml, 5g fun awọn igo reagent 250-500ml, 10g fun awọn igo reagent 500-1000ml, ati 20g fun awọn igo reagent 1000-2000ml.
Itu ti awọn phosphoramidites yẹ ki o ṣe labẹ afẹfẹ inert, ati rirọpo ti awọn reagents activator ati acetonitrile yẹ ki o pari ni akoko.Awọn ohun elo Capping ati Oxidation yẹ ki o lo ni kete bi o ti ṣee, awọn reagents ti o ṣii fun igbesi aye selifu kere, ati iṣẹ ṣiṣe ti o dinku lakoko iṣelọpọ.
Akoko ifiweranṣẹ: Oṣu Kẹjọ-09-2022